BioMachines Nanoscopy Lab

Just out!

A guide to single-particle tracking. F Simon, LE Weiss, S van Teeffelen. Nature Reviews Methods Primers (2024).
https://www.nature.com/articles/s43586-024-00341-3
https://doi.org/10.1038/s43586-024-00341-3
- Experimental and analysis guide -

"The nuclear basket regulates the distribution and mobility of nuclear pore complexes in budding yeast," J Zsok, F Simon, G Bayrak  L Isaki, N Kerff, Y Kicheva, A Wolstenholme, LE Weiss, E Dultz. Molecular Biology of the Cell (2024).
doi.org/10.1091/mbc.E24-08-0371
- Single-molecule tracking -

Coming soon

Monitoring DNA double-strand break repair in Saccharomyces cerevisiae at high throughput and high resolution. Y Shalev Ezra, A Saguy, G Levin, LE Weiss, O Alalouf, Y Shechtman. BioRxiv (2024).
doi.org/10.1101/2024.08.23.609327
- 3D imaging flow cytometry -
- PSF engineering -

Vortex light field microscopy: 3D spectral single-molecule imaging with a twist. Zhang et al. BiorXiv (2024, Accepted @Optica). doi.org/10.1101/2024.07.18.604091
- Localization in 4D (x, y, z, λ) at video framerates -

Detecting directed motion and confinement in single-particle trajectories using hidden variables. F Simon et al. eLife (2024). doi.org/10.7554/eLife.99347.1
- Operates on single tracks or populations of tracks -
- Classifies motion type and performs statistical likelihood tests  -

Large-scale visualisation of α-synuclein oligomers in Parkinson's disease brain tissue. Andrews, Fu, & Toomey et al. BioRxiv (2024).
doi.org/10.1101/2024.02.17.580698
- Co-localization -
- High-throughput pipeline -

Lab Fun