BioMachines Nanoscopy Lab

The Weiss lab is an interdisciplinary group in the Department of Engineering Physics at Polytechnique Montreal. We develop tools that have broad applications ranging from fundamental biophysics to biomedical diagnostics.

Le labo Weiss est un groupe interdisciplinaire du Département de génie physique de Polytechnique Montréal. Nous développons des outils qui ont de larges applications allant de la biophysique fondamentale au diagnostic biomédical.

An animation reading Weiss Lab in bioluminescent fluid.

A bioluminescent media flowing through a microfluidic channel (Thanks Omer!)

published in 2022!

Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Adir et al. Nature Communications. Open access 10.1038/s41467-022-29871-8

- RNA and protein synthesizing synthetic cells -
- Using chemiluminescence to trigger self activate protein interactions -
- Capable of communicating with living cells using light -

Quantifying cell cycle dependent chromatin dynamics during interphase by live 3D tracking. Naor et al., iScience. Open access doi.org/10.1016/j.isci.2022.104197

- 3D tracking of fluorescent telomeres inside fibroblasts -
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Sub-diffusive behavior changes with cell cycle -
- Behavior is anisotropic, xy versus z -

High-throughput Imaging of CRISPR- and Recombinant Adeno-associated Virus–induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells. Allen & Weiss et al., CRISPR Journal Open access 10.1089/crispr.2021.0128

- CRISPR-Cas9 promiscuity correlates with the DNA damage response (DDR) amplitude and dynamics -
- A larger DDR by imaging was observed for gRNAs with higher total CRISPR-Cas9 editing -
- Imaging flow cytometry characterization of rAAV6 treatments for minimizing downstream toxic effects -